A THOROUGH EVALUATION OF BIOMARKERS IN BRAZILIAN WOMEN WITH TNBC UNDERGOING NEOADJUVANT CHEMOTHERAPY – TNBCBIOMARKERS

Rationale: Locally Advanced Triple-Negative Breast Cancers (LATNBCs) are tumors with a high rate of relapse and death, and there is a strong need to explore reliable social and pathological biomarkers. Based on molecular profile, TNBC have been classified into five subtypes with different immunohistochemical profile. Methods: This is a single-institutional, retrospective, observational and translational cohort performed through TMAs of women with LACMTN enrolled at INCA between January 2010 and December 2014. PRIMARY Objectives: To evaluate the association of the TILs status with complete pathological response (cPR) and PFS. SENCODARY Objectives: 1. To determine the prevalence of a large immunohistochemical panel (AR, EGFR, CK5/6, CK14, CK17, CD 117, p53, Ki67 level, PD-L1 and PD-L2 in tumor cell membrane and the pattern of tumor infiltrating mono-lymphocytes PD-1+, FOXP3+, CD 4+ , CD8 +, CD 3+, CD56+, CD68+ and/or CD 14+), before and after chemo 3. To evaluate association between socio-demographic variables with OS, DFS and cPR. Results: Herein we present some descriptive sociodemographic data. 236 patients were enrolled, mean age 49.68 (SD ± 11.66), caucasians (47.03%), schooling under 8 years (44.06%), living at a mean distance of 36.12 Km (SD ± 42.9) from the cancer center. Predominantly grade 3 (60.16%), cT3-4 (86.44%), lymph node positive (74.5%), clinical stage III (85.16%). The vast majority (94.04%) of the patients were exposed to anthracycli and taxane-based regimen, mean of 6.97 cycles (SD ± 1.21). As complementary treatment, 10 patients received platinum (mean 3 cycles, SD ± 0.20), 6 were exposed to capecitabine (mean 3 cycles, SD ± 1.60) and 11 patients submitted to radiotherapy. Radical surgery was performed in 230 (97.45%) women and axillary dissection in 205 (86.86%). The mean time to onset of treatment was 62.29 days (SD ± 58.04), mean duration of treatment, 140.2 days (SD ± 38.58) and mean time to end of treatment for surgery, 82.67 days (SD ± 180.06). The CPR rate was 20.76%. Of the 236 patients, 92 (38.98%) relapsed and 85 (36.01%) died to date. Discussion: The lower rate of pCR compared to other reports can be explained by the late diagnosis and the prolonged interval between the diagnosis and treatment. Pathological data are still under final analysis, planned to be presented at this conference. We will present unpublished thorough evaluation of TILs and how it impacts on cPR and survival outcomes.